Troponin-Tropomyosin Complex

Troponin-Tropomyosin Complex COLUMN CHROMATOGRAPHIC SEPARATION AND ACTIVITY OF THE THREE ACTIVE TROPONIN COMPONENTS WITH AND WITHOUT TROPOMYOSIN

By means of new or modified procedures troponin-tropomyosin complex was fractionated into three components plus tropomyosin, and then reconstituted in the absence of urea. Tropomyosin was separated from troponin by hydroxyapatite column chromatography, a method giving sharper separation than the previously used technique of isoelectric precipitation. The chromatography also separated the tropom...

Troponin-tropomyosin complex. Column chromatographic separation and activity of the three, active troponin components with and without tropomyosin present.

By means of new or modified procedures troponin-tropomyosin complex was fractionated into three components plus tropomyosin, and then reconstituted in the absence of urea. Tropomyosin was separated from troponin by hydroxyapatite column chromatography, a method giving sharper separation than the previously used technique of isoelectric precipitation. The chromatography also separated the tropom...

Troponin-tropomyosin abnormalities in hamster cardiomyopathy.

Cardiac myofibrils from cardiomyopathic hamsters exhibit elevated Mg2+ ATPase activity and a parallel upward shift of the calcium ATPase dose response curve. To explore the mechanism, myofibrils from control and cardiomyopathic hamster hearts were incubated with isolated troponin-tropomyosin complex (Tn.Tm) from cardiomyopathic and control hamster or from dog hearts. Tn.Tm from control hamster ...

The role of the calcium-troponin-tropomyosin complex in the activation of contraction.

Cooperative binding of myosin subfragment-1 to the actin-troponin-tropomyosin complex.

The binding of myosin subfragment-1 (S-1) to the F-actin-troponin-tropomyosin complex (regulated F-actin) was examined in the presence of ADP (ionic strength, 0.23 M; 22 degrees C) by using the ultracentrifuge and S-1 blocked at SH1 with iodo[14C]acetamide. S-1 . ADP binds with positive cooperativity to regulated F-actin, both in the presence and absence of calcium; it binds independently to un...